Enzyme Action Labs

Enzyme(# of yeast drops)

To start off, my class played a game to try and get a feel for what an enzyme action does. By having the enzymes chase the glucose and capture it each time and then later adding inhibitors and enzyme the less glucose there was.
Lab Group: Brett, Me, Josh, and Sam
We later started a lab in which we'd be testing the amount of pressure the yeast had on the other materials in the test tube. Our first lab we started by adding 3ml of 3%  H2O2 and 3ml of water in to 3 different test tubes. To not mess up our lab results we would conduct each separately. We first added 10 drops of the mixed yeast into test tube one and put the gas pressure sensor inside the tube. 3 minutes of the pressure would be measured each tube and our computer would keep track of the rising pressure of the gas. Once 3 minutes had passed we took of the gas pressure sensor and received 8.007 kPa for 10 drops. After taking of the gas pressure sensor we added 15 yeast mix drops into the 2nd test tube. After putting on the gas pressure sensor on our second test tube we let the 3 minutes pass and let the computer record our data.Our data showed that the 15 drops of yeast had the pressure of 11.4 kPa. Finally we'd be test 20 drops of yeast mix. 20 drops was put into the 3rd test tube and had the gas pressure sensor put into it. 3 minutes of watching the line incline the data showed 8.583 kPa. My bar graph shows that the pressure was highest when there was 15 drops of yeast put into the H2O2 and water. The tube with only 10 drops came out with a pressure of 8.007 kPa, which is the lowest pressure in this experiment.

Enzyme( temperature)

The next day we tested the pressure on 20 drops of yeast in different types of temperature. We first filled 4 test tubes with H2O2 and water then placed each tube into a different temperate area. Placing one in ice, one in hot water, once at room temperature, and in warm water. The most reasonable part would be to start with the room temperature because we don't have to wait for the water to heat up or cool down. So we added 20 drops of yeast and then put the gas pressure sensor on and measured its pressure for 3 minutes. Room temperature shows 8.587 kPa.  After about 5-10 minutes we recieved the cold water and added its 20 drops of yeast and started to measure its gas temperature. 3 minutes later it showed 6.037 kPa. Next was the warm water. Adding yeast then letting the pressure of the gas be collected into the computer, we then came out with 7.427 kPa. Finally, hot water and 20 yeast drops was tested for the pressure it had and was recorded onto the computer. The hot water's pressure came out to 7.427 kPa. According to my results, the room temperature had the highest pressure than the cold, warm, and hot water.

Enzyme( Ph)

This time in the lab we will be testing the pressure of the Ph and yeast together. In each of the test tubes we added H2O2 and then the Ph. First we did the neutral ph and the pressure wiht the gas pressure sensor. After the 3 minutes had passed we collected our data. The neutral turned out with a pressure of 6.4 kPa. Repeating this same step for the acidic and basic Ph, the acidic ph pressure came out as 5.316 kpa and the basic ph pressure was 6.805.Analyzing the data collect, it showed that the basic ph had the highest pressure of the experiment with a range of 1.489 between the highes basic ph(6.805 kpa) and the lowest acidic(5.316 kPa).